Nitrogen Evaporation Technique in Metabolic Extraction: European Molecular Biology Laboratory

Small molecule chemicals found within a biological sample, known as a metabolome, can change rapidly. According to the European Molecular Biology Laboratory (EMBL), sample preparation is one of the most critical parts of a successful metabolomics experiment.  

Researches across the globe are using Organomation instruments in their metabolomics research.

EMBL, Europe’s flagship laboratory for basic research in molecular biology, demonstrates a general protocol of extraction of polar metabolites from a HeLa cell culture.

The drying step utilizes Organomation’s 24 position MICROVAP small vial evaporator in this video tutorial: https://youtu.be/QThSKVXG7qM.

This general protocol can be adapted for other samples considering the following: type of biological matrix, sample collection, quenching of cellular metabolism, extraction and recovery of metabolites, drying, storage, and shipment.

For more information about the European Molecular Biology Laboratory and protocols for research in metabolomics, please visit EMBL.org.